Using RNA extracted from mMEC, MFG or SC represents a more accessible method for assessing gene expression in the mammary epithelium4, particularly when dynamic studies require the repeated analysis of samples from the same animal. Considering the cost and labor involved to obtain a sample of mammary parenchyma for RNA extraction, analyzing milk SC and MFG appears to be an alternative, direct and simple approach that provides a high correlation with gene expression in MGT and LCMEC without the need to perform a biopsy of the mammary gland tissue. Our results present the similarities between different sampling methods by examining the transcriptome of mammary gland tissue and different RNA sources from milk.
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